The SGLT-2 Inhibitor Dapagliflozin Has a Therapeutic Effect on Atherosclerosis in Diabetic ApoE^−/− Mice

Figure 6

Effect of dapagliflozin on the macrophages was investigated with different stimulations. BMDMs from WT were treated with LPS, LPS+PA, LPS+HG, or LPS+PA+HG for 24 h and then with dapagliflozin and ATP for 30 m and tested the level of IL-1 in the supernatant (a). Collecting the cell lysates and testing the expression of NLRP3 and caspase-1 with Western blot. Data show mean ± SD; . ; versus LPS. versus LPS+dapa (b). The WT and NLRP3^−/− mice macrophages were isolated, cultured, and stimulated with LPS, LPS+PA, LPS+HG, or LPS+PA+HG for 24 h and then assayed the IL-1β in supernatant (c) and detected the expression of NLRP3 and caspase-1 in cell lysates with Western blot. Data show mean ± SD; . ; versus WT+LPS (d).