CD226 Deficiency Alleviates Murine Allergic Rhinitis by Suppressing Group 2 Innate Lymphoid Cell Responses

Figure 4

CD226 is inducible on nasal mucosal ILC2s. (a) Gating strategy for the identification of ILC2s (Lin⁻ CD45⁺ ST2⁺ KLRG1⁺). (b) CD226 expression on isolated ILC2s from the nasal mucosa of control and AR mice using FCM assay. (c) CD226 expression in the nasal mucosa of control and AR mice using qPCR assay (). The mice were sensitized against OVA and challenged with OVA at regular intervals; the control mice were sensitized and challenged with only saline. AR: allergic rhinitis; OVA: ovalbumin; NS: normal saline; ILC2s: type 2 innate lymphoid cells; FCM: flow cytometry; qPCR: quantitative real-time PCR; WT: wild type; KO: knockout; .

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