Research Article
High-Fat Mouse Model to Explore the Relationship between Abnormal Lipid Metabolism and Enolase in Pancreatic Cancer
Figure 6
The cellular level confirmed that high-lipid upregulated enolase, accelerated the process of EMT, and aggravated the malignant behavior of pancreatic cancer cells. (a) PANC-1 cells were treated with different concentrations of oleic acid, and lipid formation was examined by oil Red O staining. (b, c) PANC-1 cells were stimulated with 100 nM oleic acid to detect cell migration and invasion. (d–f) WB was used to detect the expression of enolase-related indicators ENO1/ENO2/ENO3 and EMT-related indicators E-cadherin/N-cadherin/vimentin/α-SMA. (g, h) The expression of enolase-related indicators ENO1/ENO2/ENO3 was detected by immunofluorescence. Expression of EMT-related genes E-cadherin/N-cadherin.
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