lnc-MRGPRF-6:1 Promotes ox-LDL-Induced Macrophage Ferroptosis via Suppressing GPX4

Figure 1

ox-LDL-induced ferroptosis in macrophage. (a–e) The relative expression of MDA (a), ROS (b), LDH (c), GSH (d), IRON (e) in macrophage. (f) Cell viability of macrophage. (g) Images of oil red O staining. (h) Mitochondrial morphology was observed by TEM. Scale bar: 500 nm. The data were expressed as mean with standard deviation (n = 3). , , , and vs. control cells. ^####, ^###, ^##, and ^# vs. ox-LDL-treated cells. ox-LDL, oxidized-low-density lipoproteins; Fer-1, ferrostatin-1; TEM, transmission electron microscopy. LDH, lactate dehydrogenase; MDA, malondialdehyde; GSH, glutathione; ROS, reactive oxygen species. Control, untreated macrophages; ox-LDL: macrophages treated with 50 mg/L ox-LDL for 24 hr. ox-LDL + Fer-1: macrophages co-treated with 50 mg/L ox-LDL and 5 μmol/L Fer-1 for 24 hr. Erastin: macrophages treated with 5 μM Erastin for 24 hr.

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