lnc-MRGPRF-6:1 Promotes ox-LDL-Induced Macrophage Ferroptosis via Suppressing GPX4

Figure 2

Role of lnc-MRGPRF-6:1 in THP-1-derived macrophage ferroptosis. (a, b) The mRNA level of lnc-MRGPRF-6:1. (c–g, i–m) The relative expression of MDA (c, i), ROS (d, j), LDH (e, k), GSH (f, l), and SOD (g, m). (h, n) Cell viability. The data were expressed as mean with standard deviation (n = 3). , , , and . 50 mg/L ox-LDL was used to stimulate macrophage for 24 hr. Control, negative control lentivirus-infected macrophages; KD, lnc-MRGPRF-6:1 knockdown macrophages; OV, lnc-MRGPRF-6:1 overexpression of macrophages. MDA, malondialdehyde; ROS, reactive oxygen species; LDH, lactate dehydrogenase; GSH, glutathione; SOD, superoxide dismutase.

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