lnc-MRGPRF-6:1 Promotes ox-LDL-Induced Macrophage Ferroptosis via Suppressing GPX4

Figure 4

lnc-MRGPRF-6:1 may regulate macrophage ferroptosis via GPX4. (a) KEGG classification analysis between lnc-MRGPRF-6:1 knockdown macrophage and control. (b) KEGG enrichment analysis of between lnc-MRGPRF-6:1 knockdown macrophage and control. (c) The heat map of differential genes involved in ferroptosis between control and KD. (d) The mRNA expression level of GPX4 in macrophage. (e) The protein expression level of GPX4 in macrophage. The data were expressed as mean with standard deviation (n = 3). , , and . Macrophages were cultured with 5 μM RSL-3 for 24 hr to inhibit the expression of GPX4. Control, negative control lentivirus-infected macrophage; KD, lnc-MRGPRF-6:1 knockdown macrophage; KEGG: Kyoto Encyclopedia of Genes and Genomes. GPX4, glutathione peroxidase 4; SLC7A11, solute carrier family 7 member 11; ACSL4, Acyl-CoA synthase long-chain 4; ACSL3, Acyl-CoA synthase long-chain 3; FTH, ferritin heavy chain; FTL, ferritin light chain; NCOA4, nuclear receptor coactivator 4; TFRC, transferrin receptor; RSL-3, RAS-selective lethal compounds 3.

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