circRNA_17725 Promotes Macrophage Polarization towards M2 by Targeting FAM46C to Alleviate Arthritis

Figure 7

circRNA_17725 functioned as a ceRNA based on the circRNA_17725-miR-4668-5p-FAM46C network. (a) Real-time PCR: increased expression of FAM46C in Lv-circRNA-transfected Raw264.7 macrophages (; ). (b) Cell immunofluorescence: higher expression of FAM46C in Raw264.7 macrophages transfected by Lv-circRNA plasmids (representative pictures; 20x). (c) Real-time PCR: decreased miR-4668-5p in Lv-circRNA-treated cells (; ). (d) Luciferase reporter assay: miR-4668-5p mimics inhibited FAM46C expression in macrophages (; ). (e) RIP test: expression of circRNA_17725 in immunoprecipitations (; ). (f) RIP test: expression of miR-4668-5p in immunoprecipitations (; ).

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