GATA6-AS1 via Sponging miR-543 to Regulate PTEN/AKT Signaling Axis Suppresses Cell Proliferation and Migration in Gastric Cancer

Figure 2

GATA6-AS1 represses cell proliferation and migration in GC. (a) QRT-PCR was used to analyze the overexpression efficiency of pcDNA3.1/GATA6-AS1 in transfected MKN-45 and AGS cells and interference efficiency of sh-GATA6-AS1#1/#2/#3 in transfected HGC-27 cells. (b) CCK-8 assays were applied to access the cell viability when GATA6-AS1 was overexpressed or inhibited in GC cells. (c) Colony formation assays were performed to assess cell proliferation when GATA6-AS1 was upregulated or silenced in GC cells. (d) The migration ability of GC cells was assessed by wound healing assays in the indicated cells. (e) Transwell assays were further conducted to evaluate cell migration after the overexpression or deficiency of GATA6-AS1 in GC cells. (f–h) Representative image, tumor growth curve, and tumor weight at the end points of xenografted tumors formed by subcutaneous injection of MKN-45 cells stably transfected with pcDNA3.1 or pcDNA3.1/GATA6-AS1 into nude mice. The number of nude mice used in each group is 3. Student’s -test for overexpression studies and one-way ANOVA and Dunnett’s test for knockdown studies. .

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