Inhibition of Schwann Cell Pyroptosis Promotes Nerve Regeneration in Peripheral Nerve Injury in Rats

Figure 3

Ac-YVAD-cmk attenuated Schwann cell pyroptosis. (a) A CCK-8 assay was used to measure the viability of Schwann cells treated with various concentrations of Ac-YVAD-cmk (10, 30, and 50 μM). compared with the control group. ^# compared with the LPS+ATP group. Data are expressed as the . (b, c) IL-18 and IL-1β levels in the culture supernatant determined by ELISAs. compared with the control group. ^# compared with the LPS+ATP group. Data are expressed as the . (d) NLRP3, N-GSDMD, and Cleaved-casp-1 levels were detected by western blot analysis. (e–g) Levels of NLRP3, N-GSDMD, and Cleaved-casp-1 were quantified by densitometric measurement. compared with the control group. ^# compared with the LPS+ATP group. Data are expressed as the . (h) Schwann cells were incubated with GSDMD and DiI after treatment. Immunofluorescence analysis of the distribution of GSDMD in the membranes of Schwann cells induced by LPS and ATP treatment and then exposed to different concentrations of the pyroptosis inhibitor Ac-YVAD-cmk (10, 30, and 50 μM). Scale bar, 100 μm.

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