Neutrophil-Derived IL-6 Potentially Drives Ferroptosis Resistance in B Cells in Lupus Kidney

Figure 2

Neutrophil-derived IL-6 conducted a major signal between neutrophils and B cells in lupus kidney. (a) Bar plots showed the global communications (interaction numbers and strength) between neutrophils and B cells in control kidney and in lupus kidney. (b) Circular plots showed inferred signal numbers among neutrophil (Ly6g), neutrophil (Aldh2), neutrophil (Socs2), and B cells, in control and lupus kidney, respectively. (c) Dot plots showed outgoing and incoming inferred interaction strength among neutrophil (Ly6g), neutrophil (Aldh2), neutrophil(Socs2), and B cells, in control and lupus kidneys, respectively. (d) Dot plot showed neutrophil- (Ly6g) derived IL-6 was the major signal targeting on B cells. (e) Flow cytometry analysis validated neutrophil (Ly6g) was the major source of IL-6 in lupus kidney. Dead cells were removed by counterstaining fixable viability dye. (f) Dot plots showed the statistical analysis of resource of upregulated IL-6 from 3 neutrophil subclusters in lupus kidney (), compared with control kidney (). Data represents similar results from at least 3 independent experiments. ; ns: no significance.

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