p16^INK4a Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin

Figure 1

High fat diet induces p16 overexpression and accumulation in skin senescent cells. Eight-week-old ApoE^-/- mice were fed with normal diet (ND) and High fat diet (HFD) for 3 months and obtained skin tissues for further analysis. (a) H&E staining showing inflammatory infiltration of skin from 20-week-old ApoE^-/- mice induced by ND and HFD (); (b) representative images of Masson staining to assess skin collagen deposition from 20-week-old ApoE^-/- mice induced by ND and HFD (); (c–f) expression levels and statistical figures of α-SMA, Collagen I, and Collagen III in skin tissues from 20-week-old ApoE^-/- mice induced by ND and HFD were detected by western blotting (); (g–i) expression levels of α-SMA, Collagen I, Collagen III, and p16 in skin tissues from 20-week-old ApoE^-/- mice induced by ND and HFD by immunohistochemical staining (); (j–l) Expression levels and statistical figures of p16 and p53 in skin tissues from 20-week-old ApoE^-/- mice induced by ND and HFD by western blotting (); (m, n) expression levels and statistical figures of p16 in skin tissues from 20-week-old ApoE^-/- mice induced by ND and HFD by immunohistochemical staining (); values are , , , compared with ND mice.

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