p16^INK4a Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin

Figure 2

High fat diet induces inflammaging in skin of ApoE^-/- mice. Eight-week-old ApoE^-/- mice were fed with normal diet (ND) and High fat diet (HFD) for 3 months and obtained skin tissues for further analysis. (a–d) Expression levels and statistical figures of Bcl-2, Bax, and cleaved-caspase-3 in skin tissues from 20-week-old ApoE^-/- mice induced by ND and HFD by western blotting (); (e, f) representative images and statistical analysis of Caspase-3 in skin by immunohistochemical staining (); (g, h) representative images and statistical analysis of IL-1β, IL-6, and TNF-α in skin by immunohistochemical staining (); (i, j) expression levels and statistical figures of MMP9, MMP3, pro-IL-1β, mature-IL-1β, TNF-α, and IL-6 in skin tissues from 20-week-old ApoE^-/- mice induced by ND and HFD by western blotting (); (k, l) expression levels and statistical figures of pp65(S536) and p65 in skin tissues from 20-week-old ApoE^-/- mice induced by ND and HFD by western blotting (); values are , , , , compared with ND group mice.

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