p16^INK4a Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin

Figure 3

Steatosis induced or p16 overexpression aggravates inflammaging phenotypes in human dermal fibroblasts. Human dermal fibroblasts (HDFs) were induced steatosis for 24 h under medium containing sodium palmitate (10 mmol/L) and sodium oleate (10 mmol/L); HDF cells were transfected with NC and p16 overexpression adenovirus for further analysis. (a, b) Expression levels and statistical figures of p16 in skin fibroblasts and induced steatosis of skin fibroblasts by western blotting (); (c, d) expression levels and statistical figures of α-SMA, Collagen I, Collagen III, and p16 in skin fibroblasts transfected with NC and p16 overexpression adenovirus by western blotting; (e) representative images of Oil Red staining in skin fibroblasts transfected with NC and p16 overexpression adenovirus (); (f, g) expression levels and statistical figures of p19, β-galactosidase in skin fibroblasts transfected with NC, and p16 overexpression adenovirus; (h, i) expression levels and statistical figure of IL-1β, IL-6, and TNF-α in skin fibroblasts transfected with NC and p16 overexpression adenovirus by western blotting; (j, k) expression levels and statistical figures of p65, pp65(S536) in skin fibroblasts transfected with NC, and p16 overexpression adenovirus by western blotting (); values are , , , compared with NC group.

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