p16^INK4a Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin

Figure 4

Transcriptomics identify the inhibitory effects of proinflammatory responses in the skin of p16 knockout mice. Eight-week-old ApoE^-/- and ApoE^-/-p16^-/- were fed with HFD for 12 weeks and obtained skin tissues for further analysis. (a) Pattern diagram of mice experiment; (b) H&E staining of skin from 20-week-old ApoE^-/- and ApoE^-/-p16^-/- mice induced by HFD (); (c) representative images of Masson staining to assess skin collagen deposition from 20-week-old ApoE^-/- and ApoE^-/-p16^-/- mice induced by HFD (); (d–f) expression levels and statistical figures of Collagen I and α-SMA in skin tissues from 20-week-old ApoE^-/- and ApoE^-/-p16^-/- mice induced by HFD by western blotting (); (g) RNA-seq analysis on HFD-induced skin (), identified 1150 statistically significant () differentially expressed genes which were presented as a volcano plot: fold change from ApoE^-/- vs. ApoE^-/-p16^-/-; (h) differentially expressed genes by RNA-seq were shown as Heatmap; (i, j) functional profiling shown Biological Process of all differentially expressed genes identified by RNA-seq using Panther Classification System; (k) representative protein–protein interaction (PPI) network built with all downregulation significantly altered genes using STRING. Further functional analysis was performed with DAVID and significantly enriched categories are highlighted; values are , , compared with ApoE^-/- mice.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)