p16^INK4a Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin

Figure 5

HFD or p16 overexpression activates the NLRP3 inflammasome pathway. Eight-week-old ApoE^-/- mice were fed with normal diet (ND) and high fat diet (HFD) for 3 months and obtained skin tissues for further analysis. (a, b) Expression levels and statistical figures of ASC, Caspase-1, Caspase-1 p10, and NLRP3 in skin tissues from 20-week-old ApoE^-/- mice induced by ND and HFD by western blotting (); (c–h) expression levels and statistical analysis of NLRP3, ASC, and Caspase-1 in skin tissues from 20-week-old ApoE^-/- mice induced by ND and HFD by immunohistochemical staining (); (i–l) human dermal fibroblasts (HDF) were induced steatosis for 24 h under medium containing sodium palmitate (10 mmol/L) and sodium oleate (10 mmol/L); HDF cells were transfected with NC and p16 overexpression adenovirus for further analysis. Expression levels and statistical figures of Caspase-1, ASC, and NLRP3 in skin fibroblasts transfected with NC and p16 overexpression adenovirus by western blotting (); values are , , , , compared with ND or NC group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)