p16^INK4a Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin

Figure 6

p16 knockout ameliorated activation of integrin-inflammasome pathway induced by high fat diet. (a, b) Further functional analysis of all upregulation differentially expressed genes from ApoE^-/- and ApoE^-/-p16^-/- was performed with GO analysis and significantly enriched categories are highlighted; (c, d) expression levels and statistical figure of ASC, ITGAL, ITGAM, NLRP3, and NLRC4 in skin tissues from 20-week-old ApoE^-/- and ApoE^-/-p16^-/- mice induced by HFD by western blotting (); (e, f) expression levels and statistical figures of TNF-α, mature-IL-1β, IL-6, MMP3, β-Galactosidase, and p19 in skin tissues from 20-week-old ApoE^-/- and ApoE^-/-p16^-/- mice induced by HFD by western blotting (); (g, h) expression levels and statistical analysis of ASC, Caspase-1, and NLRP3 in skin tissues from 20-week-old ApoE^-/- and ApoE^-/-p16^-/- mice induced by HFD by immunohistochemical staining (); (i) graphic abstract shown that p16 accumulation induced by HFD promoted activation of integrin-inflammasome pathway, thus aggravating accumulation of senescent cells and inflammaging in skin; values are , , compared with ApoE^-/- mice.

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