Dietary High-Fat Promotes Cognitive Impairment by Suppressing Mitophagy

Figure 4

Expression of proteins related to mitophagy and mitochondrial morphology. (a) Schematic diagram of the mitophagy. (b, c) The western blotting indicated that HFD reduced the expression of PINK1, Parkin, Beclin1, and LC3II and increased the expression of p62 in the brains of aged C57BL/6 mice (, two-sample -test). (d, e) The expression of proteins related to mitophagy in N2a cells is treated with PA medium (200 μM). This expression profile was in line with the in vivo results (, one-way ANOVA). Data are shown as , , , , and . (f) The mitochondrial structure in the hippocampal cytoplasm in the ND group was intact and clear. However, some neurons in the HFD group were necrotic, and most mitochondria in the cytoplasm appeared mildly swollen. N: nucleus; Mi: mitochondria. . Abbreviations: ND: normal diet; HFD: high-fat diet; CON: control; PA: palmitic acid; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

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