EGCG Protects against 6-OHDA-Induced Neurotoxicity in a Cell Culture Model

Table 1

Effect of 6-OHDA and EGCG on the iron-related gene expression (mean ± SEM, = 3–6).

	6-OHDA	EGCG + 6-OHDA	p
DMT1 + IRE	2.3 ± 0.2	0.9 ± 0.0	<0.001
Hepcidin	1.7 ± 0.1	0.8 ± 0.2	<0.01
Fpn1	0.7 ± 0.0	1.2 ± 0.2	<0.05
H-ferritin	2.4 ± 0.4	1.1 ± 0.2	<0.05
TfR1	0.7 ± 0.0	1.3 ± 0.1	<0.01
TfR2	1.5 ± 0.1	1.1 ± 0.1	<0.05

N27 cells were treated with 25 µM of 6-OHDA for 24 h following pretreatment by EGCG 100 µM for 2 h. The values are ratios compared to the control after individual mRNA values were normalized to GAPDH expression. p values indicate the difference between the 6-OHDA and the EGCG + 6-OHDA treatment groups.
; ns: not significant compared to control.
Differences were determined using Student’s -test.