Protective Role of AMPK against PINK1^B9 Flies’ Neurodegeneration with Improved Mitochondrial Function

Figure 2

AMPK expression and target protein in AMPK pathway detected in AMPK overexpressed PINK1^B9 flies. (a) AMPK mRNA expression confirmation in MHC-Gal4 drove AMPK interfered flies (n ≥ 3/group). (b, c) Immunoblot of the indicated genotypes of flies (n ≥ 10/group) with the antibody specific to the phosphorylated form of p-AMPK-α. β-Actin serves as a loading control. (d) Drosophila line generating strategy. (e–h) Immunoblot of the indicated genotypes of flies (n ≥ 10/group) with the antibody specific to the phosphorylated form of AMPK α, total-AMPK, and β-actin serves as a loading control. (i, j) Immunoblot of the indicated genotypes of flies (n ≥ 10/group) with the antibody specific to the phosphorylated form of ULK1 and α-tubulin serves as a loading control. Data are shown as the mean ± S.D. (, , , , ns = not significant).

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