Protective Role of AMPK against PINK1B9 Flies’ Neurodegeneration with Improved Mitochondrial Function

<div>AMPK overexpression suppresses PINK1B9 flies’ neurodegeneration. (a) Whole brains of 10-day-old male flies (n ≥ 5/group) driven by TH-gal4 of the indicated genotypes were stained with antityrosine hydroxylase (TH) antibody. Panel shows confocal images of DA neuron of the PPL1 cluster of the indicated genotypes. (b) Quantification of the PPL1 neurons in brains of the indicated genotypes (n ≥ 3/group) in each condition. Bar graph shows the number of PPL1 cluster dopaminergic neurons. (c) Whole-fly brain lysates (n ≥ 10/group) were analyzed by western blot analysis by using the tyrosine hydroxylase (TH) antibody. (d) Relative tyrosine hydroxylase (TH) protein expression in the brains (n ≥ 3/group). Values were expressed as mean ± S.D. 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Parkinson’s Disease

fig4

Figure 4

Figure 4: Protective Role of AMPK against PINK1B9 Flies’ Neurodegeneration with Improved Mitochondrial Function