The Neuroprotective Effect of Isotetrandrine on Parkinson’s Disease via Anti-Inflammation and Antiapoptosis In Vitro and In Vivo

Figure 3

Effect of ITD on 6-OHDA-induced modulation of inflammation-related mRNA expression in zebrafish. Zebrafish larvae were pretreated with 200 μM ITD from 9 hpf to 5 dpf and then challenged with 250 μM 6-OHDA from 2 to 5 dpf and harvested at 5 dpf for qPCR. (a–d) Quantitative PCR results of tyrosine hydroxylase (th1), inos, cox-2, and tumor necrosis factor-α (tnf-α) in each group. Data are presented as mean ± SEM. Each sample contained pooled material from twenty fish. significantly different from the control group; ^#significantly different from the 6-OHDA group, .

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