PPAR-γ Activation Alleviates Osteoarthritis through Both the Nrf2/NLRP3 and PGC-1α/Δψ_m Pathways by Inhibiting Pyroptosis

Figure 4

Piog partially attenuates LPS/ATP-induced chondrocyte pyroptosis related to the NLRP3 inflammasome through Nrf2 signaling. (a and b) Western blot analysis for NLRP3, caspase-1 P10, and GSDMD-N in the control, LPS/ATP, Piog + LPS/ATP, and Piog + MSU + LPS/ATP groups. (b) ELISA was performed for IL-1β and IL-18 concentrations. (c) CCK8 assays were performed to determine the effect of Piog on LPS/ATP-treated chondrocyte viability after MSU activated NLRP3. (d) Western blot analysis for Nrf2 in the control, LPS/ATP, Piog, and Piog + LPS/ATP groups. (e) Western blot analysis was performed to detect NLRP3 level after Nrf2 knockdown by siRNA; (f) ELISA was performed to detect IL-1β and IL-18 concentrations. (g) CCK8 assays were performed to determine the effect of Piog on LPS/ATP-treated chondrocyte viability after Nrf2 knockdown by siRNA. GAPDH was used as an internal reference for western blotting. Densitometric analysis of the immunoblots is located on the right or below. The data represent three independent experiments and are expressed as mean ± SD. * and **.

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