Buspirone Induces Weight Loss and Normalization of Blood Pressure via the Stimulation of PPARδ Dependent Energy Producing Pathway in Spontaneously Hypertensive Rats

Figure 5

Western blot analyses for PPARδ, p-AMPK, and PGC-1α in the abdominal fat tissues of SHRs treated with buspirone of 1, 5, or 10 mg/kg/day (0.01, 0.05, or 0.1 mg/mL/kg/day). PPARδ expression was increased in groups treated with buspirone of 1, 5, and 10 mg/kg/day (0.01, 0.05, and 0.1 mg/mL/kg/day) compared to 8w untreated group (a). Phosphorylated AMPK level was not significantly elevated in groups treated with buspirone 1, 5, and 10 mg/kg/day (0.01, 0.05, and 0.1 mg/mL/kg/day) (b). PGC-1α protein expression was increased in groups treated with buspirone of 1, 5, and 10 mg/kg/day (0.01, 0.05, and 0.1 mg/ml/kg/day) (c). The results are expressed as mean ± standard error of the mean (). Values were statistically analyzed using the unpaired t-test to compare two groups, and to compare means of three or more groups was used one-way ANOVA test and followed by Bonferroni’s multiple comparisons test. All experiments were repeated over three times. *, **.

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