Research Article

Appraisal of the Possible Role of PPARγ Upregulation by CLA of Probiotic Pediococcus pentosaceus GS4 in Colon Cancer Mitigation

Figure 3

Biohydrogenation-derived CLA of probiotic GS4 induced PPARγ in HCT-116 cells: The HCT-116 cells were pre-incubated for 1 hour with/without BADGE (20 μM) before treatment of CLA/CLAGS4 for 48 hour. The PPARγ expression was examined by western blot analysis. The GAPDH was used as loading control. The densitometric analysis of bands was evaluated using the ImageJ software. Data (means ± SE) were statistical analysed by one way-ANOVA with post hoc Newman–Keuls analysis and depicted as *versus respective control.