Research Article
Appraisal of the Possible Role of PPARγ Upregulation by CLA of Probiotic Pediococcus pentosaceus GS4 in Colon Cancer Mitigation
Figure 4
The biohydrogenation-derived CLA altered down effectors of PPARγ in HCT-116. The HCT-116 cells were pre-incubated for 1 hour with/without BADGE (20 μM) before treatment of CLA/CLAGS4 for 48 hour. (a) The COX-2 and 5-LOX expression were studied by western blot analysis. GAPDH was used as a loading control. (b) The densitometric analysis of bands was evaluated using the ImageJ software version 1.49. (c) The PGE2 level in HCT-116 cells. Data (means ± SE) and were statistical analysed () by one way-ANOVA with post hoc Newman–Keuls analysis and depicted as *versus control; #versus BADGE control.
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