Comparative Study of Spermatogenesis and Nucleolar Behavior in Testicular Lobes of Euschistus heros (Heteroptera: Pentatomidae)
Figure 2
Meiotic cells stained with lacto-acetic orcein ((a)–(i)) and silver impregnated ((j)–(p)). ((a)–(c)) Early prophase I with heteropyknotic body in the periphery of the nucleus (arrows), with (a) belonging to lobes 1–3, (b) to 4 and 6 and (c) to 5; (d) diplotene/diakinesis showing a bivalent with interstitial chiasma (arrowhead) and heteropyknotic body (arrow); (e) final stage of diakinesis showing X and Y chromosome, respectively, arrow and arrowhead; ((f), (g)) anaphase/telophase I with regular segregation of chromosomes; (h) metaphase II showing the autosomes arranged in ring-shape and the sex chromosomes in the center; (i) anaphase/telophase II with lagging migration of the X chromosome (arrow) and the Y chromosome in the opposite part of the cell (arrowhead); ((j)–(l)) early prophases I showing two round silver-positive bodies (arrows) that are disorganized during the subsequent phases and one body less impregnated (arrowheads). Note that in prophase of lobes 4 and 6 (k), there are several silver-positive bodies scattered throughout the nucleus; (m), (n)) metaphase I with silver impregnation in the cytoplasm and chromosomes (m), differing from lobe 5, which has silver-negative metaphase (n); ((o), (p)) telophase II with nucleolar reorganization in both cell formations (arrows). Bar = 10 m.
