Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells

Figure 4

Expression of markers of adipogenesis. RNA was isolated from cells differentiated in media supplemented with 10% FBS or 10% hPL, and expression of PPARG, ADIPOQ, GLUT4, and LEP was measured by RT-PCR. Statistically significant differences were determined by one-way ANOVA with Tukey’s multiple comparisons. Data represents the mean ± SEM of three biological replicates from three donors/group. RB represents the mean ± SEM of three biological replicates from one donor from a commercial source. Significant differences between groups are designated by alphabetical letters. Any groups with the same letter are not statistically different from each other (, Tukey’s MCT).