Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells

Figure 8

Assessment of alkaline phosphatase activity in osteogenic differentiated and control undifferentiated ASCs. ALP activity was assessed in cells under cultured control (CTRL) and osteogenic conditions (OSTEO) in media supplemented with 10% FBS or 10% hPL, then normalized to cellular protein content by BCA. Data are expressed as ALP activity (U/mg protein) in undifferentiated and differentiated cells at day 7 (a) and day 14 (b) and as fold change over control conditions following 7 and 14 days osteogenic differentiation (c). Statistically significant differences were determined by one-way ANOVA with Tukey’s multiple comparisons. Data represent the mean ± SEM of three biological replicates from three donors/group. RB represents the mean ± SEM of three biological replicates from one donor from a commercial source. Significant differences between groups are designated by alphabetical letters. Any groups with the same letter are not statistically different from each other (, Tukey’s MCT).

(a) ALP Activity U/mg Protein Day 7

(b) ALP Activity U/mg Protein Day 14

(c) ALP Activity U/mg Protein Fold Change