Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells

Figure 9

Light micrographs of Alizarin red S staining. ASCs bought from a commercial vendor (RB) were compared to ASCs derived from abdominoplasty (HAP) or burned human skin (BH) cultured in αMEM with 10% FBS and 10% hPL and differentiated under osteogenic conditions with identical supplements for 14 days, then stained to assess mineralization. Representative images of undifferentiated RB (a), HAP cells (b, c), and BH cells (d, e) from control media conditions and differentiated RB (f), HAP cells (g, h), and BH (i, j) are given. White scale bar represents 100 μm.