Fetal Dermal Mesenchymal Stem Cell-Derived Exosomes Accelerate Cutaneous Wound Healing by Activating Notch Signaling

Figure 3

FDMSC exosomes regulate proliferation, migration, and secretion of ADFs. (a) The internalization of exosomes by ADFs. (b) FDMSC exosomes can significantly stimulate ADF proliferation in a dose-dependent manner. (c, d) Transwell assay of the migration of ADFs in different exosome-treatment groups. (c) Cell migration is expressed as a percentage of control. (d) Representative images of the migration (×100 magnification). Cells were counted for at least five random microscope fields. Results are shown as from three independent experiments. (e) FDMSC exosomes promote ECM protein secretion of ADFs. Real-time PCR analysis of the mRNA levels of Type I and III collagen, elastin fibronectin, and α-SMA genes (normalized to GAPDH) in ADFs of different groups. Results are shown as of three independent experiments. Two-tailed Student’s -test was used. , , or .

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