Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells

Figure 1

ASCs exhibited broad-spectrum antibacterial activity for both gram-positive and gram-negative strains. Several bacterial strains were brought in contact for 6 hours without (blank bars) or with (black bars) ASCs aerobically or anaerobically. (a) Bacteria were stained Syto-62® and propidium iodide (PI), then analyzed by flow cytometry. Changes in the proportion of PI-positive bacteria reflected changes in bacterial wall permeability (). (b) After serial dilutions, bacteria were incubated onto agar for CFU counting with or without ASCs (). (c) Scatter graph represented changes in CFU number versus proportion of PI-positive bacteria. A linear regression was represented with the corresponding correlation coefficient. (d) After labelling with DiOC6(3), the green/red ratio was used to analyze modification of bacterial membrane polarization independently from the change of bacterial size and shape (). , , and between conditions with and without ASCs.

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