Interleukin-1β Enhances Umbilical Cord Mesenchymal Stem Cell Adhesion Ability on Human Umbilical Vein Endothelial Cells via LFA-1/ICAM-1 Interaction

Figure 1

IL-1β induces increased expression of LFA-1 in MSCs. (a) MSCs without treated IL-1β as a control group and treated with IL-1β for 15, 30, 120, and 360 minutes (scale bar: 50 μm). Histograms analyze LFA-1 (green) fluorescence intensity and location of one single MSC. White blocks show a single MSC. Red lines show the histogram detection sites. Results of measurement of LFA-1 (green) and cell nucleus (blue) fluorescence intensity are quantified by MetaMorph. (b) Example of Western blot results of the LFA-1 (129 kDa) expression in cytosolic and membrane fractions. Cells were treated with IL-1β for 15, 30, and 120 minutes. (c) Quantitative graphs of the Western blot results of LFA-1 protein expression of (b) (, ). (d) Example of Western blot results of the LFA-1 (129 kDa) from cell membrane protein. Cells treated with IL-1β and IL-1RA or cotreated with IL-1β and IL-1RA. (e) Quantitative graphs of the Western blot results of LFA-1 protein expression of (d) (, , and ).

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