Interleukin-1β Enhances Umbilical Cord Mesenchymal Stem Cell Adhesion Ability on Human Umbilical Vein Endothelial Cells via LFA-1/ICAM-1 Interaction

Figure 2

IL-1β-induced ICAM-1 expression in HUVECs and enhanced MSC adhesion to HUVECs via LFA-1/ICAM-1. (a) Immunocytochemistry staining of ICAM-1 (green) and cell nucleus (blue) in HUVECs treated with or without IL-1β for 15, 30, 120, and 360 minutes (scale bar: 50 μm). Histograms analyze ICAM-1 (green) fluorescence intensity and location of one single MSC. White blocks show a single MSC. Red lines show histogram detection sites. Results of measured ICAM-1 (green) and cell nucleus (blue) fluorescence intensity are quantified by MetaMorph. (b) Representative image of the adhesion of MSCs to HUVECs. MSCs were treated with IL-1β, IL-1RA, and adhesion to IL-1β-activated HUVECs or nonactivated HUVECs. MSCs labelled with calcein AM (5 μM) (green), HUVECs, and MSC cell nuclei were stained with Hoechst 33258 (blue) (scale bar: 50 μm). (c) Quantitative graphs of the cell adhesion assay results of MSC adhesion to nonactivated HUVECs (black bars) or IL-1β-activated HUVECs (gray bars). Values were the cell number fold change relative to the control group. Data represent (, , and ) (N.S.: nonsignificance). (d) Representative image of MSC adhesion on HUVECs. Cell adhesion assay to determine the percentage of MSC adhesion on the HUVEC monolayer. MSC adhesion to IL-1β-activated HUVECs or nonactivated HUVECs after treatment with IL-1β and the inhibitor lovastatin. (e) Quantitative graphs of the cell adhesion assay results of MSC adhesion to nonactivated HUVECs (black bars) or IL-1β-activated HUVECs (gray bars). MSCs treated with IL-1β and the inhibitor lovastatin. Data represent (,, , and ).

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