Human Adipose-Derived Hydrogel Characterization Based on In Vitro ASC Biocompatibility and Differentiation

Figure 4

Analysis of osteogenic differentiation of ASCs in DAT hydrogel. ASC seeded DAT hydrogels (2 × 10⁶ cells/mL) were cultured in stromal or osteogenic media for 14 or 28 days and analyzed by qRT-PCR (a, b) and Von Kossa staining (c, d). (a, b) Expression of osteogenic genes in osteoegnically induced ASC samples after 14 (a) or 28 days (b) of culture is displayed as fold change relative to the control ASC samples. (c, d) Representative images of Von Kossa stained histological sections of Control ASC (c) and osteogenic ASC (d) seeded hydrogels. (d) Mineral deposits (brown) are indicated by arrows. Data are expressed as mean () ± SD; level of significance: () ; Scale bar 200 μm.

(a)

(b)

(c)

(d)