Human Adipose-Derived Hydrogel Characterization Based on In Vitro ASC Biocompatibility and Differentiation

Figure 5

Analysis of ASC and DAT hydrogel fiber morphology using images acquired by SEM. (a-d) Representative SEM images of DAT hydrogel (no cells) (a), control ASC (b), adipogenic ASC (c), and osteogenic ASC (d) seeded DAT hydrogels are displayed at 3 magnifications; 1,000X (Arrows indicate cells; Scale bar 50 μm), 2,000X (magnified to display cell morphology; Scale bar 20 μm), and 50,000X (magnified to display fiber morphology; Scale bar 1 μm). Fiber morphology metrics were measured from 50,000X images using ImageJ software (diamterJ plugin) to determine mean fiber diameter (e) mean pore size (f), and fiber intersections/ μm² (g). Data are expressed as mean () ± SD; level of significance: () ; () ; () .