Rheological and Biological Properties of Adhesive Skin Secretions from Eupsophus vertebralis (Anura: Alsodidae)

Figure 4

Cytotoxicity assays for Eupsophus vertebralis secretions using the MG63 cell line: (a) cell viability of indirect cytotoxicity tests using an extract of the secretion, following ISO 10993-5 standard, and (b) cell viability of direct cytotoxicity tests, where cells were in direct contact with the secretion (see text for details). Cell viability is shown at 24 h for direct tests and 24 and 48 h for indirect tests. The red line indicates 70% viability.

(a)

(b)