Noncovalent Interactions of Tiopronin-Protected Gold Nanoparticles with DNA: Two Methods to Quantify Free Energy of Binding

Table 2

Solubilities (𝑆), equilibrium binding constants (𝐾^1-PyCHO/DNA), and the corrected free energy (Δ𝐺^CORR) corresponding to 1-PyCHO/DNA interaction at different Au@tiopronin concentrations.

10^−6 [AuNPs]/M	𝑆/M	10^−4𝐾^1-PyCHO/DNA/M^−1	(Δ𝐺^CORR)/kJmol^−1
0.0	1.21×10^−6	78.0	−27.91
2.5	3.02×10^−6	77.0	−25.59
6.0	1.21×10^−5	58.1	−21.47
10.0	2.41×10^−5	49.8	−19.39
20.0	2.49×10^−5	46.0	−19.11