Optimization of Aeration and Agitation Rate for Lipid and Gamma Linolenic Acid Production by Cunninghamella bainieri 2A1 in Submerged Fermentation Using Response Surface Methodology

Table 6

Analysis of variance for the quadratic polynomial model of GLA content produced by Cunninghamella bainieri 2A1.

Source	Polynomial coefficients	Sum of squares	DF	Mean square	value	Prob >
Model			5		27.81	0.0002^**
Intercept	0.49
			1		22.14	0.0022^**
			1		53.93	0.0002^**
			1		7.66	0.0278^*
			1		39.46	0.0004^**
			1		10.43	0.0145^*
Residual			7
Lack of fit			3		3.97	0.1081
Pure error			4

Statistically significant at 95% probability level.
^**Statistically significant at 99% probability level.
: aeration rate (vvm); : agitation rate (rpm); and : the quadratic terms; : the interaction term.
= 0.9521.