Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination

Figure 1

ZBves expression in the eyes of transgenic line. The Tol2 transposon system was established to produce a Tg(zbves : EGFP) transgenic zebrafish line. The zbves promoter sequences were cloned in the Tol2-containing vector (a). The F3 transgenic embryos were observed using a confocal microscope to investigate the expression pattern of Bves. Before 18 hpf, the GFP signal (transgene) mainly appeared on the surface of whole organism and eye (b)–(d). At 18 hpf, Bves expression initially spread into the retina from the border between the cornea and retina (e), (e′). At 24 hpf, zBves was expressed in whole retina of the zebrafish eye (f), (f’). At 96 hpf, zBves was localized around IPL and RPE (g), (g′), and (g′′). Antibody staining with Bves was preformed in 24-hpf, 96-hpf, and adult fish (h), (i), and (i′). The lateral side of the eye with phalloidin staining (red) is shown in (e′), (f′), (g′), and (g′′). Scale bar, 50 μm.