Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination

Figure 4

Adherens junctions were not formed in the outer limiting membrane of zbves morphants. Immunostaining of adherens junction-related molecules-ZO-1 (a)–(d), aPKC (e)–(h), and β-catenin (i)–(l)—was carried out in the retinas of wild-type and zBves MO-injected embryos and zbvesmRNA rescued morphants at 72 hpf. In wild-type embryos, β-catenin obviously appeared in the apical site of photoreceptors (i), whereas it disappeared in zbve morphants (j), (k). The defect in this protein among morphants could be rescued by coinjection with zbve mRNA. Arrows indicate the apical edge. Nuclei were counterstained with DAPI (blue). Scale bar, 10 μm.

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