Aquaporin-1 Expression in Proliferative Vitreoretinopathy and in Epiretinal Membranes
Figure 1
AQP1 mRNA expression in membranes from PVR and ERM. First Column: cDNAs from pools of 3 PVR membranes or 6 ERM membranes were used for RT-qPCR. The amplification curves obtained with cDNA from a positive control (mallow curve), a negative control (red curve), PVR (green curve), and ERM (light blue curve) are shown for each tested gene. Second Column: RT-qPCR amplification curves obtained with cDNA from a positive control (light blue curve) or 2.5 ng genomic DNA (gDNA) (black curve) are shown for each tested gene primers. Third Column: RT-qPCR melt curves obtained following 40 RT-qPCR cycles and with cDNA from a positive control (mallow curve), PVR (green curve), and ERM (light blue curve) or genomic DNA (red curve) are shown for each tested gene.