Aquaporin-1 Expression in Proliferative Vitreoretinopathy and in Epiretinal Membranes

Figure 3

AQP1 expression colocalized with αSMA and GFAP. First row of images represents the negative control (a–c), respectively, in green, red, and blue (DAPI stains the cell nuclei). (d) AQP1 expression in green; (e) αSMA expression in red; (f) AQP1 and αSMA colocalisation (merge of (d) and (f) images) and DAPI; (g) AQP1 expression in green; (h) GFAP expression in red; (i) AQP1 and GFAP colocalisation (merge of (g) and (h) images) and DAPI; (j) GFAP expression in green; (k) αSMA expression in red; (l) lack of GFAP and αSMA colocalisation (merge of (j) and (k) images) and DAPI. (f′), (i′), and (l′) represent a high magnification of the white square, respectively, drawn in the (f), (i), and (l) picture. Empty arrowheads designate single marker (AQP1 or αSMA or GFAP), while solid arrow point out coexpression of AQP1/αSMA (f′) or AQP1/GFAP (i′). There was no colocalisation between GFAP and αSMA in the (l′) picture. Empty arrowheads designate cells labelled by a single marker, either GFAP in green or αSMA in red (l′). Scale bars represent 50 µm.