Culture and Characterization of Microglia from the Adult Murine Retina

Figure 1

Effects of different culture media on the yield and the morphology of the cultured microglial cells. (a) Mixed retinal culture grown in DMEM showing low cell numbers. ((b) and (d)) Cells grown in DMEM/F12 + Glutamax showing a good yield and no change in the morphology was observed. ((c) and (e)) Some of the cells grown in RPMI/F12 + Glutamax started to change into more round-shaped cells. Microglial cells were observed after 15 days in culture using a phase-contrast Olympus microscope and Prog Res C14 imaging software. Magnification ×20; (d) and (e) are enlarged. Data are representative of 3 experimental repeats.