Culture and Characterization of Microglia from the Adult Murine Retina
Figure 5
Immunofluorescent staining of cultured retinal microglial cells. Microglial cells were transferred into a 16-well chamber slide and stained for (a) isotype control (insert, phase contrast image to show the cells), (b) F4/80, (c) CD11b, (d) CD45, (e) MHC-II (insert, phase contrast), (f) CD86 (insert, phase contrast), and (g) CD40 (insert, phase contrast). The samples were analysed by confocal microscopy. In all images blue indicates allophycocyanin (APC). Over 98% of the cells were positive for CD11b, CD45, and F4/80 surface markers but the staining of CD45 was much weaker compared to CD11b and F4/80. Cells were negative for MHC-II, CD86, and CD40. Data were representative of 2 experiments.
