Qualitative and Quantitative Analysis of the Major Constituents in Chinese Medical Preparation Lianhua-Qingwen Capsule by UPLC-DAD-QTOF-MS
Table 1
Quantitative results of 12 compounds in LQC extracted by different methods.
Content (g/g)
Methods
30%a
60%
90%
60%
60%
60%
60%
60%
60%
30 minb
30 min
30 min
30 min
30 min
30 min
15 min
30 min
45 min
1 : 100c
1 : 100
1 : 100
1 : 50
1 : 100
1 : 200
1 : 100
1 : 100
1 : 100
Salidroside
1726.28
1701.25
1622.02
1522.31
1701.25
1656.32
1711.54
1701.25
1688.54
Chlorogenic acid
2444.97
2492.15
2216.89
2285.43
2492.15
2289.27
2492.62
2492.15
2552.17
Forsythoside E
1583.93
1620.78
451.91
1462.09
1620.78
1402.53
1627.01
1620.78
1579.63
Cryptochlorogenic acid
1862.98
1851.64
667.50
1703.05
1851.64
1771.79
1837.89
1851.64
1857.95
Amygdalin
1424.11
1455.39
1442.56
1268.93
1455.39
1298.11
1395.97
1455.39
1431.38
Sweroside
816.19
813.18
789.32
747.06
813.18
772.67
812.51
813.18
808.24
Hyperin
135.10
151.73
167.26
140.84
151.73
140.80
152.72
151.73
157.67
Rutin
122.00
121.17
115.22
106.11
121.17
116.67
117.09
121.17
116.62
Forsythoside A
2484.60
2536.34
2661.79
2285.76
2536.34
2396.35
2543.87
2536.34
2521.10
Phillyrin
1660.26
1521.45
1551.59
1410.19
1521.45
1390.12
1523.01
1521.45
1551.52
Rhein
803.13
1102.06
1370.11
937.63
1102.06
932.05
956.38
1102.06
1054.77
Glycyrrhizic acid
1530.49
1680.43
1594.37
1437.40
1680.43
1619.99
1674.81
1680.43
1665.42
Extracting solvent: 30%, 60%, and 90% methanol-water solution.
bUltrasonic time: 15 min, 30 min, and 45 min.
cExtraction solvent multiples: 1 : 50, 1 : 100, and 1 : 200 expressed 50, 100, and 200 times per gram of sample.