Research Article
Qualitative and Quantitative Analysis of the Major Constituents in Chinese Medical Preparation Lianhua-Qingwen Capsule by UPLC-DAD-QTOF-MS
Table 3
Linear regression, LODs and LOQs, intraday and interday precisions, repeatability, stability, and recovery for 12 compounds.
| Compound |
Regression equationa () | |
Linear range (g/mL) |
LODb (μg/mL) |
LOQc (μg/mL) |
Intraday (RSD, %) () |
Interday (RSD, %) () | Repeatability () | Stability (RSD, %) | Recovery ( = 6) | Mean (g/g) | RSD (%) | Original (g) | Spiked (μg) | Detected (μg) | Recovery (%) | RSD (%) |
| Salidroside | | 0.9999 | 5.27–84.35 | 1.06 | 3.50 | 0.12 | 0.26 | | 0.61 | 1.38 | 355.91 | 354.20 | 707.50 | 99.28 | 1.24 |
| Chlorogenic acid | | 0.9998 | 6.85–109.56 | 1.71 | 5.69 | 0.77 | 2.12 | | 0.51 | 2.54 | 494.74 | 493.85 | 959.90 | 94.19 | 2.42 |
| Forsythoside E | | 0.9995 | 4.85–77.64 | 1.21 | 4.04 | 0.67 | 2.25 | | 1.07 | 2.67 | 351.81 | 351.05 | 677.26 | 92.99 | 3.54 |
| Cryptochlorogenic acid | | 0.9997 | 6.65–106.47 | 1.66 | 5.53 | 1.10 | 1.63 | | 1.02 | 2.46 | 405.90 | 406.60 | 784.00 | 93.10 | 1.43 |
| Amygdalin | | 0.9998 | 3.91–62.57 | 0.98 | 3.26 | 0.79 | 0.93 | | 1.74 | 2.48 | 297.08 | 298.62 | 576.39 | 93.58 | 3.32 |
| Sweroside | | 0.9999 | 2.00–31.96 | 0.25 | 0.80 | 0.11 | 0.33 | 798.06 ± 2.93 | 0.37 | 0.48 | 159.61 | 159.51 | 325.43 | 103.95 | 0.41 |
| Hyperin | | 0.9999 | 0.20–3.21 | 0.051 | 0.16 | 0.78 | 0.62 | 92.03 ± 0.80 | 0.87 | 0.83 | 18.41 | 18.24 | 37.07 | 102.29 | 1.82 |
| Rutin | | 0.9993 | 0.53–8.50 | 0.072 | 0.21 | 0.41 | 2.24 | | 1.66 | 1.57 | 36.83 | 36.81 | 72.50 | 96.90 | 2.34 |
| Forsythoside A | | 0.9999 | 4.21–67.34 | 0.30 | 1.05 | 0.20 | 2.92 | | 0.74 | 1.95 | 497.00 | 495.95 | 997.33 | 100.89 | 1.49 |
| Phillyrin | | 0.9999 | 2.86–45.71 | 0.71 | 2.36 | 0.79 | 1.63 | | 0.64 | 2.76 | 315.56 | 316.72 | 623.99 | 100.25 | 1.60 |
| Rhein | | 0.9996 | 3.47–55.49 | 0.87 | 2.88 | 0.40 | 0.28 | | 2.39 | 0.97 | 136.38 | 136.27 | 273.48 | 100.61 | 1.61 |
| Glycyrrhizic acid | | 0.9999 | 5.27–84.35 | 0.092 | 0.33 | 0.34 | 2.09 | | 1.08 | 2.15 | 324.55 | 323.939 | 652.16 | 101.31 | 3.27 |
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is the peak area; is the concentration of standard solutions.
bLOD refers to the limits of detection, .
cLOQ refers to the limits of quantity, .
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