Research Article
Chemical Profiling and Biological Activities on Nepalese Medicinal Plant Extracts and Isolation of Active Fraction of Nyctanthes arbor-tristis
Table 6
Isolation of active fractions from the extract of Nyctanthes arbor-tristis.
| Eluting solvent system | Fraction no | Volume of eluent (mL) | TLC solvent system | TLC report |
| 100% hexane | 1–3 | 300 | 1% EtOAc in hexane | No spot | 100% hexane | 4–6 | 300 | 1% EtOAc in hexane | No spot | 100% hexane | 7–10 | 400 | 1% EtOAc in hexane | No spot | 1% EtOAc in hexane | 11–13 | 300 | 2% EtOAc in hexane | No spot | 1% EtOAc in hexane | 14–16 | 300 | 2% EtOAc in hexane | No spot | 3% EtOAc in hexane | 17–20 | 400 | 5% EtOAc in hexane | No spot | 3% EtOAc in hexane | 21–24 | 400 | 5% EtOAc in hexane | Tailing | 5% EtOAc in hexane | 24–28 | 500 | 10% EtOAc in hexane | One spot (tailing) | 5% EtOAc in hexane | 29–33 | 500 | 10% EtOAc in hexane | Tailing | 5% EtOAc in hexane | 34–38 | 500 | 10% EtOAc in hexane | Tailing | 10% EtOAc in hexane | 39–42 | 400 | 10% EtOAc in hexane | Tailing | 10% EtOAc in hexane | 43–46 | 400 | 10% EtOAc in hexane | Tailing | 15% EtOAc in hexane | 47–51 | 500 | 20% EtOAc in hexane | No clear spot | 25% EtOAc in hexane | 52–54 | 300 | 30% EtOAc in hexane | Two clear spots | 25% EtOAc in hexane | 55–58 | 400 | 30% EtOAc in hexane | No distinct spot | 40% EtOAc in hexane | 59–61 | 300 | 40% EtOAc in hexane | Tailing | 40% EtOAc in hexane | 62–64 | 300 | 40% EtOAc in hexane | Tailing | 60% EtOAc in hexane | 65–67 | 300 | 70% EtOAc in hexane | Tailing | 60% EtOAc in Hexane | 68–70 | 300 | 70% EtOAc in Hexane | Multi-spots | 80% EtOAc in hexane | 71–74 | 400 | 40% EtOAc in hexane | Tailing | 100% EtOAc | 75–81 | 600 | 1% MeOH in EtOAc | Tailing | 1% MeOH in EtOAc | 82–86 | 500 | 2% MeOH in EtOAc | Single spot | 3% MeOH in EtOAc | 87–91 | 500 | 5% MeOH in EtOAc | One spot (tailing) | 5% MeOH in EtOAc | 92–96 | 500 | 10% MeOH in EtOAc | Single spot (tailing) | 10% MeOH in EtOAc | 97–101 | 500 | 15% MeOH in EtOAc | One spot (tailing) | 15% MeOH in EtOAc | 102–105 | 400 | 25% MeOH in EtOAc | No clear spot | 25% MeOH in EtOAc | 106–110 | 500 | 30% MeOH in EtOAc | Tailing | 50% MeOH in EtOAc | 111–115 | 500 | 60% MeOH in EtOAc | Tailing | 75% MeOH in EtOAc | 116–120 | 500 | 80% MeOH in EtOAc | Tailing | 100% MeOH | 121–128 | 700 | 60% MeOH in EtOAc | Tailing |
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