Antibodies Induced by Lipoarabinomannan in Bovines: Characterization and Effects on the Interaction between Mycobacterium avium Subsp. paratuberculosis and Macrophages In Vitro
Figure 2
(a) Characterization of LAM extract by SDS-PAGE (lane 1: purified LAM of M. tuberculosis, lane 2: LAM extract, both silver-stained for carbohydrate detection) and immunoblot. (lane 3: LAM extract reactivity assessed against one representative serum from the infected control group). (b) Immunoblot of bovine sera reactivity against LAM extract. Left side of the dotted line: LAM extract was run as antigen. D and UD indicate protein-digested or undigested antigen. 1, 2 and 3 show results of three representative sera from LAM-immunized group. Normal control shows results representative for sera in that group. Right side of the dotted line: ovalbumin (OVA) was run as antigen and reactivity assessed against serum of an OVA-immunized bovine, as a positive control of protein digestion. MWM: molecular weight markers.
