The Effects of Substituting Glassware for Plasticware and the Use of an Ethanol Vector on Oocyte Maturation In Vitro

Figure 1

Blastomere nuclear spread (cytoplasm dissolved) stained for cell counting and DNA fragmentation (TUNEL). Apoptosis is detected by green FITC-labeled signals (b, e, h) colocalized with red PI-labeled DNA (a, d, g). Overlay of FITC and PI (c, f, i). Negative control (a, b, c) with strong PI staining for DNA, 100x magnification. Positive control (d, e, f) with strong FITC staining for induced DNA breaks, 200x magnification. Example of treatment staining (g, h, i), 200x magnification. Arrow (i): overlay of red and green signal with fragmentation characteristic of apoptosis.

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