The Effects of Substituting Glassware for Plasticware and the Use of an Ethanol Vector on Oocyte Maturation In Vitro
Figure 2
PCR results for embryo sex determination. Example of multiplexed PCR using TSPY and GAPDH. (a) and (b) lane 1: water control with some primer-dimer accumulating below 100 bp. (a) lane 2: TSPY at 1100 bp from a positive control bull testicle sample. (b) lane 2: GAPDH at 260 bp from positive control cattle ovarian tissue. (c) Three males (lanes 1, 2 and 4) and one female (lane 3). All treatment samples are day eight embryos. All ladders are 100 bp ladders.
